Integrated photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for multimodal chorioretinal imaging

Chao Tian; Wei Zhang; Van Phuc Nguyen; Ziyi Huang; Xueding Wang; Yannis M. Paulus

doi:10.1117/12.2290594

19 February 2018 Integrated photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for multimodal chorioretinal imaging

Chao Tian, Wei Zhang, Van Phuc Nguyen, Ziyi Huang, Xueding Wang, Yannis M. Paulus

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104945U (2018) https://doi.org/10.1117/12.2290594
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Current clinical available retinal imaging techniques have limitations, including limited depth of penetration or requirement for the invasive injection of exogenous contrast agents. Here, we developed a novel multimodal imaging system for high-speed, high-resolution retinal imaging of larger animals, such as rabbits. The system integrates three state-of-the-art imaging modalities, including photoacoustic microscopy (PAM), optical coherence tomography (OCT), and fluorescence microscopy (FM). In vivo experimental results of rabbit eyes show that the PAM is able to visualize laser-induced retinal burns and distinguish individual eye blood vessels using a laser exposure dose of ~80 nJ, which is well below the American National Standards Institute (ANSI) safety limit 160 nJ. The OCT can discern different retinal layers and visualize laser burns and choroidal detachments. The novel multi-modal imaging platform holds great promise in ophthalmic imaging.

Citation Download Citation

Chao Tian, Wei Zhang, Van Phuc Nguyen, Ziyi Huang, Xueding Wang, and Yannis M. Paulus "Integrated photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for multimodal chorioretinal imaging", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104945U (19 February 2018); https://doi.org/10.1117/12.2290594

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