Development towards compact nitrocellulose interferometric biochips for dry eye diagnosis based on MMP9, S100A6 and CST4 biomarkers using a Point-of-Care device

Beatriz Santamaría; María Fe Laguna; David López-Romero; A. López-Hernandez; F. J. Sanza; A. Lavín; R. Casquel; M. Maigler; M. Holgado

doi:10.1117/12.2288257

19 February 2018 Development towards compact nitrocellulose interferometric biochips for dry eye diagnosis based on MMP9, S100A6 and CST4 biomarkers using a Point-of-Care device

Beatriz Santamaría, María Fe Laguna, David López-Romero, A. López-Hernandez, F. J. Sanza, A. Lavín, R. Casquel, M. Maigler, M. Holgado

Author Affiliations +

Proceedings Volume 10474, Ophthalmic Technologies XXVIII; 1047424 (2018) https://doi.org/10.1117/12.2288257
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

A novel compact optical biochip based on a thin layer-sensing BICELL surface of nitrocellulose is used for in-situ labelfree detection of dry eye disease (DED). In this work the development of a compact biosensor that allows obtaining quantitative diagnosis with a limited volume of sample is reported. The designed sensors can be analyzed with an optical integrated Point-of-Care read-out system based on the “Increase Relative Optical Power” principle which enhances the performance and Limit of Detection. Several proteins involved with dry eye dysfunction have been validated as biomarkers. Presented biochip analyzes three of those biomarkers: MMP9, S100A6 and CST4. BICELLs based on nitrocellulose permit to immobilize antibodies for each biomarker recognition. The optical response obtained from the biosensor through the readout platform is capable to recognize specifically the desired proteins in the concentrations range for control eye (CE) and dry eye syndrome (DES). Preliminary results obtained will allow the development of a dry eye detection device useful in the area of ophthalmology and applicable to other possible diseases related to the eye dysfunction.

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Beatriz Santamaría, María Fe Laguna, David López-Romero, A. López-Hernandez, F. J. Sanza, A. Lavín, R. Casquel, M. Maigler, and M. Holgado "Development towards compact nitrocellulose interferometric biochips for dry eye diagnosis based on MMP9, S100A6 and CST4 biomarkers using a Point-of-Care device", Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 1047424 (19 February 2018); https://doi.org/10.1117/12.2288257

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