Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)

Samira Aghayee; Dan Winkowski; Patrick Kanold; Wolfgang Losert

doi:10.1117/12.2191538

5 October 2015 Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)

Samira Aghayee, Dan Winkowski, Patrick Kanold, Wolfgang Losert

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Proceedings Volume 9548, Optical Trapping and Optical Micromanipulation XII; 95481P (2015) https://doi.org/10.1117/12.2191538
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

The spatial connectivity of neural circuits and the various activity patterns they exert is what forms the brain function. How these patterns link to a certain perception or a behavior is a key question in neuroscience. Recording the activity of neural circuits while manipulating arbitrary neurons leads to answering this question. That is why acquiring a fast and reliable method of stimulation and imaging a population of neurons at a single cell resolution is of great importance. Owing to the recent advancements in calcium imaging and optogenetics, tens to hundreds of neurons in a living system can be imaged and manipulated optically. We describe the adaptation of a multi-point optical method that can be used to address the specific challenges faced in the in-vivo study of neuronal networks in the cerebral cortex. One specific challenge in the cerebral cortex is that the information flows perpendicular to the surface. Therefore, addressing multiple points in a three dimensional space simultaneously is of great interest. Using a liquid crystal spatial light modulator, the wavefront of the input laser beam is modified to produce multiple focal points at different depths of the sample for true multipoint two-photon excitation.

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Samira Aghayee, Dan Winkowski, Patrick Kanold, and Wolfgang Losert "Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)", Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481P (5 October 2015); https://doi.org/10.1117/12.2191538

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KEYWORDS

Neurons

Wavefronts

Cerebral cortex

Imaging systems

Optical manipulation

Phase modulation

Brain

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