CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)

Nisan Siegel; Brian Storrie; Marc Bruce; Gary Brooker

doi:10.1117/12.2081319

11 March 2015 CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)

Nisan Siegel, Brian Storrie, Marc Bruce, Gary Brooker

Author Affiliations +

Proceedings Volume 9336, Quantitative Phase Imaging; 93360S (2015) https://doi.org/10.1117/12.2081319
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

FINCH holographic fluorescence microscopy creates high resolution super-resolved images with enhanced depth of focus. The simple addition of a real-time Nipkow disk confocal image scanner in a conjugate plane of this incoherent holographic system is shown to reduce the depth of focus, and the combination of both techniques provides a simple way to enhance the axial resolution of FINCH in a combined method called "CINCH". An important feature of the combined system allows for the simultaneous real-time image capture of widefield and holographic images or confocal and confocal holographic images for ready comparison of each method on the exact same field of view. Additional GPU based complex deconvolution processing of the images further enhances resolution.

Citation Download Citation

Nisan Siegel, Brian Storrie, Marc Bruce, and Gary Brooker "CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)", Proc. SPIE 9336, Quantitative Phase Imaging, 93360S (11 March 2015); https://doi.org/10.1117/12.2081319

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