Raman microspectroscopic study of oral buccal mucosa

Isha Behl; Hitesh Mamgain; Atul Deshmukh; Lekha Kukreja; Arti R. Hole; C. Murali Krishna

doi:10.1117/12.2033933

17 March 2014 Raman microspectroscopic study of oral buccal mucosa

Isha Behl, Hitesh Mamgain, Atul Deshmukh, Lekha Kukreja, Arti R. Hole, C. Murali Krishna

Proceedings Volume 8940, Optical Biopsy XII; 89400D (2014) https://doi.org/10.1117/12.2033933
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Oral cancer is the most common cancer among Indian males, with 5-year- survival-rates of less than 50%. Efficacy of Raman spectroscopic methods in non-invasive and objective diagnosis of oral cancers and confounding factors has already been demonstrated. The present Raman microspectroscopic study was undertaken for in-depth and site-specific analysis of normal and tumor tissues. 10 normal and 10 tumors unstained sections from 20 tissues were accrued. Raman data of 160 x 60 μm and 140 x 140 μm in normal and tumor sections, respectively, were acquired using WITec alpha 300R equipped with 532 nm laser, 50X objective and 600 gr/mm grating. Spectral data were corrected for CCDresponse, background. First-derivitized and vector-normalized data were then subjected to K-mean cluster analysis to generate Raman maps and correlated with their respective histopathology. In normal sections, stratification among epithelial layers i.e. basal, intermediate, superficial was observed. Tumor, stromal and inflammatory regions were identified in case of tumor section. Extracted spectra of the pathologically annotated regions were subjected to Principal component analysis. Findings suggest that all three layers of normal epithelium can be differentiated against tumor cells. In epithelium, basal and superficial layers can be separated while intermediate layer show misclassifications. In tumors, discrimination of inflammatory regions from tumor cells and tumor-stroma regions were observed. Finding of the study indicate Raman mapping can lead to molecular level insights of normal and pathological states.

Citation Download Citation

Isha Behl, Hitesh Mamgain, Atul Deshmukh, Lekha Kukreja, Arti R. Hole, and C. Murali Krishna "Raman microspectroscopic study of oral buccal mucosa", Proc. SPIE 8940, Optical Biopsy XII, 89400D (17 March 2014); https://doi.org/10.1117/12.2033933

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available