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Effects of cryopreservation on sperm structure in Japanese pearl osyter Pinctada fucata martensii

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Abstract

To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular spermatozoa were diluted with cryopreservation diluent (10% methanol+18% fetal bovine serum+72% sea water), and dispensed into 0.25-mL straws. The straws were cooled at a rate of approximately −20 °C/min to −50°C, and subsequently immersed in liquid nitrogen. Percentage motility of spermatozoa before cryopreservation was 69.9±4.2%, and that of cryopreserved spermatozoa was 24.0±1.8%, respectively. In cryopreserved spermatozoa, the percentage that lacked or had a deformed flagellum was 56.6±3.9%, while in fresh spermatozoa this was 8.7±2.0%. In cryopreserved spermatozoa, the percentage of deformed acrosomes was 76.6±5.2%, while in fresh spermatozoa this was only 0.9±0.3%. Cryopreserved spermatozoa with a normal acrosome and flagellum were only 15.4±3.5% of those in fresh spermatozoa. These results indicate that lesion of the flagellum and deformation of the acrosome occurred through the cryopreservation procedure, and both types of damage lead to loss of the motility and fertility in thawed spermatozoa.

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Authors and Affiliations

  1. Faculty of Bioresources, Mie University, 514-8507, Tsu, Mie, Japan

    Teruyoshi Narita & Akira Komaru

  2. Department of Fisheries, Graduate School of Agriculture, Kinki University, 631-8505, Nara, Japan

    Takayuki Kawamoto & Hiromi Ohta

  3. Mie Prefectural Fish Farming Center, Hamajima, 517-0404, Shima, Mie, Japan

    Kiyoshi Isowa

  4. Fisheries Research Division, Mie Prefectural Science and Technology Promotion Center, 517-0404, Shima, Mie, Japan

    Hideo Aoki & Masahiro Hayashi

Authors
  1. Teruyoshi Narita
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  2. Takayuki Kawamoto
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  3. Kiyoshi Isowa
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  4. Hideo Aoki
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  5. Masahiro Hayashi
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  6. Hiromi Ohta
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  7. Akira Komaru
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Correspondence to Akira Komaru.

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Narita, T., Kawamoto, T., Isowa, K. et al. Effects of cryopreservation on sperm structure in Japanese pearl osyter Pinctada fucata martensii . Fish Sci 74, 1069–1074 (2008). https://doi.org/10.1111/j.1444-2906.2008.01626.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2008.01626.x

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