Skip to main content
SpringerLink
Log in

Lactic acid bacteria effective for regulating the growth of contaminant bacteria during the fermentation of Undaria pinnatifida (Phaeophyta)

  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

Lactic acid fermentation of seaweed is a recent topic and quite limited information is available on culture conditions. To know the suitable strains for use as a starter culture for seaweed fermentation, 14 lactic acid bacteria (LAB) strains, including 11 species, were tested in culture conditions prepared with or without salt. A commercial product of Undaria pinnatifida powder was used as a substrate for fermentation without sterilizing. Starter-suitability of the LAB strains was assessed from their predominance after culture. Among the tested strains, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosus showed high (>90%) predominance in their cultures, while control cultures prepared without inoculation of LAB did not show any detectable growth of acid producing bacteria and spoiled. A total of 102 strains not showing acid producing activity were isolated and characterized from spoiled cultures, and all the isolates were observed as Bacillus strains, including 64 strains (62.7%) and 16 strains (15.7%) of a Bacillus cereus-related and B. fusiformis-related species, respectively. The Undaria substrate before fermentation contained culturable microorganisms at 1.4−3.1 × 102 CFU/g, but the Bacillus cereus-related strain was not a major composition, suggesting a concern of selective growth of the Bacillus cereus-related strain during the spoiled fermentation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Steinkraus KH. Comparison of fermented foods of the East and West. In: Lee C, Steinkraus KH, Reilly PJA (eds). Fish Fermentation Technology. United Nations University Press, Tokyo. 1993; 1–12.

    Google Scholar 

  2. Uchida M, Murata M. Fermentative preparation of single cell detritus from seaweed, Undaria pinnatifida, suitable as a replacement hatchery diet for unicellular algae. Aquaculture 2002; 207: 345–357.

    Article  CAS  Google Scholar 

  3. Uchida M, Murata M. Isolation of a lactic acid bacterium and yeast consortium from a fermented material of Ulva spp. (Chlorophyta). J. Appl. Microbiol. 2004; 97: 1297–1310.

    Article  PubMed  CAS  Google Scholar 

  4. Uchida M, Numaguchi K, Murata M. Mass preparation of marine silage from Undaria pinnatifida and its dietary effect for young pearl oyster. Fish. Sci. 2004; 70: 456–462.

    Article  CAS  Google Scholar 

  5. Uchida M, Amakasu H, Satoh Y, Murata M. Combinations of lactic acid bacteria and yeast suitable for preparation of marine silage. Fish. Sci. 2004; 70: 508–518.

    Google Scholar 

  6. Uchida M, Sato Y, Murata M, Matsushima R. Characterization of the base collection strains preserved in the Marine Microorganisms Division, a sub-bank of the Microbial Culture Collections, Fish Research Agency. Bull. Fish. Res. Agent. 2004; 11: 19–30.

    Google Scholar 

  7. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenic study. J. Bacteriol. 1991; 173: 697–703.

    PubMed  CAS  Google Scholar 

  8. Dunnett CW. A multiple comparison procedure for comparing several treatments with a control. J. Am. Stat. Assoc. 1955; 50: 1096–1211.

    Article  Google Scholar 

  9. Thompson JD, Higgins DG, Gibson TJ, Clustal W. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673–4680.

    Article  PubMed  CAS  Google Scholar 

  10. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Biol. 1980; 16: 111–120.

    CAS  Google Scholar 

  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol. Biol. Evol. 1987; 4: 406–425.

    PubMed  CAS  Google Scholar 

  12. Nes IF, Diep DB, Hovarstein LS, Brurberg MB, Eijsink VGH, Holo H. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie Leeuwenhoek 1996; 70: 113–128.

    Article  PubMed  CAS  Google Scholar 

  13. Japan Collection of Microorganisms. Catalog of Strains, 9th edn. Riken Bioresource Center, Tsukuba. 2005.

    Google Scholar 

  14. Rogosa M, Genus I. Lactobacillus. In: Buchnan RE, Gibbons NE (eds) Bergey’s Manual of Determinate Bacteriology, 8th edn. Williams & Wilkins, Baltimore, 1975; 576–593.

    Google Scholar 

  15. Porter KG, Feig YS. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 1980; 5: 943–948.

    Google Scholar 

  16. Shinagawa K, Sugiyama J, Terada T, Matsusaka N, Sugii S, Improved method for purification of an enterotoxin produced by bacillus cereus. FEMS Microbiol. Lett. 1991; 64: 1–5.

    Article  PubMed  CAS  Google Scholar 

  17. Asano S, Nukumizu Y, Bando H, Iizuka T, Yamamoto T. Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 1997; 63: 1054–1057.

    PubMed  CAS  Google Scholar 

  18. Mantynen V, Lindstrom K. A rapid PCR-based DNA test for enterotoxic Bacillus cereus. Appl. Environ. Microbiol. 1998; 64: 1634–1639.

    PubMed  CAS  Google Scholar 

  19. Nakamura LK. Phylogeny of Bacillus sphaericus-like organisms. Int. J. Syst. Evol. Microbiol. 2000; 50: 1715–1722.

    PubMed  CAS  Google Scholar 

  20. Skerman VBD, McGowan V, Sneath PHA. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 1980; 30: 225–420.

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Masakazu Murata

    Present address: Nagasaki Prefectural Institute of Fisheries, Taira-cho, 851-2213, Nagasaki, Japan

Authors and Affiliations

  1. National Research Institute of Fisheries and Environment of Inland Sea, 739-0452, Hatsukaichi, Hiroshima, Japan

    Motoharu Uchida

  2. National Research Institute of Fisheries Science, 236-8648, Yokohama, Kanagawa, Japan

    Masakazu Murata

  3. Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan

    Fumiyasu Ishikawa

Authors
  1. Motoharu Uchida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masakazu Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fumiyasu Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Motoharu Uchida.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uchida, M., Murata, M. & Ishikawa, F. Lactic acid bacteria effective for regulating the growth of contaminant bacteria during the fermentation of Undaria pinnatifida (Phaeophyta). Fish Sci 73, 694–704 (2007). https://doi.org/10.1111/j.1444-2906.2007.01383.x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01383.x

Key Words

Search

Navigation