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Evaluation of genetic diversity of eight grouper species Epinephelus spp. based on microsatellite variations

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Abstract

Genetic diversity among eight species of grouper, Epinephelus bleekeri, E. coioides, E. malabaricus, E. ongus, E. akaara, E. maculatus, E. merra and E. fuscoguttatus, was studied using six microsatellite loci, Em-01 ⋆, Em-03 ⋆, Em-07 ⋆, Em-08 ⋆, Em-10 ⋆, and CA-07 ⋆, with the aim of exploring the feasibility of using microsatellite data for species identification. The results showed high levels of genetic differentiation among species (F st =0.4403 and R st =0.4954). Species identification based on fixed allelic differences was possible between E. coioides, E. ongus, and E. fuscoguttatus at Em-01 ⋆ and between E. fuscoguttatus and E. ongus at Em-08 ⋆. Private alleles were found in all species, except for E. ongus. Pairwise F st ranged 0.238–0.578 (P<0.008 Bonferroni correction), and Nei’s genetic distance ranged 0.433–2.710. Size homoplasy was observed at Em-03 ⋆ 157 allele, which was characterized by a T-C transition at the 119th nucleotide site of PCR products. The genetic assignment test unambiguously assigned each individual to the correct species. Thus, this test can be used for species identification of unknown individuals when the multilocus genotypes of the six microsatellite loci are available. The phylogenetic (neighbor-joining) tree, which was constructed based on the genetic distance matrix, separated the eight grouper species into two main groups.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, 92150, Trang, Thailand

    Worawut Koedprang

  2. Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 10900, Bangkok, Thailand

    Uthairat Na-Nakorn

  3. Laboratory of Population Genetics Informatics, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan

    Masamichi Nakajima & Nobuhiko Taniguchi

Authors
  1. Worawut Koedprang
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  2. Uthairat Na-Nakorn
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  3. Masamichi Nakajima
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  4. Nobuhiko Taniguchi
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Correspondence to Uthairat Na-Nakorn.

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Koedprang, W., Na-Nakorn, U., Nakajima, M. et al. Evaluation of genetic diversity of eight grouper species Epinephelus spp. based on microsatellite variations. Fish Sci 73, 227–236 (2007). https://doi.org/10.1111/j.1444-2906.2007.01328.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01328.x

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