Crystallization and preliminary X-ray diffraction data of the second and archaebacterial-type aspartyl-tRNA synthetase from Thermus thermophilus

The archaebacterial-type aspartyl-tRNA synthetase (AspRS2) from the thermophilic eubacterium Thermus thermophilus was crystallized using the hanging-drop vapour-diffusion method. Crystals grew at pH 9.5 in the presence of PEG 8000 and NaCl. A native diffraction data set has been collected at 2.5 Å resolution using synchrotron radiation and cryocooling. Crystals belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 57.3, b = 121.9, c = 166.9 Å and V_M = 3.03 Å³ Da^-1. There is one dimer of M_r 96 000 per asymmetric unit. A molecular-replacement analysis gave solutions for the rotation and translation functions.