Cloning, expression, purification and crystallization of saccharopine reductase from Magnaporthe grisea

The gene coding for saccharopine reductase (E.C. 1.5.1.10), an enzyme of the α-aminoadipic pathway of lysine biosynthesis in the pathogenic fungus Magnaporthe grisea, was cloned and expressed in Escherichia coli. The purified enzyme was crystallized in space groups C2 and C222₁ using ammonium sulfate pH 4.8 or PEG 6000 pH 4.1 as precipitants. The unit-cell parameters are a = 115.0, b = 56.6, c = 74.3 Å, β = 111.1° for space group C2, and a = 89.3, b = 119.0, c = 195.9 Å for space group C222₁. The crystals diffract to resolutions of 2.0 Å (C2) and 2.4 Å (C222₁) at synchrotron sources.