Model Accounting for the Effects of Pulling-Device Stiffness in the Analyses of Single-Molecule Force Measurements

Arijit Maitra and Gaurav Arya
Phys. Rev. Lett. 104, 108301 – Published 12 March 2010; Erratum Phys. Rev. Lett. 105, 259902 (2010)

Abstract

Single-molecule force spectroscopy provides a powerful approach for investigating molecular transitions along specific reaction coordinates. Here, we present a general analytical model for extracting the intrinsic rates and activation free energies from force measurements on single molecules that is applicable to a broad range of pulling speeds and device stiffnesses. This model relaxes existing limitations to perform force measurements with soft pulling devices for proper theoretical analyses and, in fact, allows experiments to specifically exploit device stiffness as a control parameter in addition to pulling speed for a reliable estimation of energetic and kinetic parameters.

  • Figure
  • Figure
  • Figure
  • Received 13 September 2009

DOI:https://doi.org/10.1103/PhysRevLett.104.108301

©2010 American Physical Society

Erratum

Authors & Affiliations

Arijit Maitra and Gaurav Arya*

  • Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0448, USA

  • *Corresponding author: garya@ucsd.edu

Article Text (Subscription Required)

Click to Expand

References (Subscription Required)

Click to Expand
Issue

Vol. 104, Iss. 10 — 12 March 2010

Reuse & Permissions
Access Options
Author publication services for translation and copyediting assistance advertisement

Authorization Required


×
×

Images

×

Sign up to receive regular email alerts from Physical Review Letters

Log In

Cancel
×

Search


Article Lookup

Paste a citation or DOI

Enter a citation
×