Topic Introduction

Optogenetics: Opsins and Optical Interfaces in Neuroscience

  1. Karl Deisseroth
Adapted from Imaging in Neuroscience (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

Optogenetics is defined as the integration of optics and genetics to control well-defined events within specified cells of living tissue. In this introduction, we focus on the basic techniques necessary for employing microbial opsins as optogenetic tools in mammalian brains. We provide a guide for the fundamentals of optogenetic application—selecting an opsin, implementing expression of opsins based on the neuroscientific experimental requirements, and adapting the corresponding optical hardware for delivery of light into mammalian brains.

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  1. doi:10.1101/pdb.top083329 Cold Spring Harb Protoc 2014: pdb.top083329- © 2014 Cold Spring Harbor Laboratory Press
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