Protocol

Dissection and Fixation of Large Milkweed Bug (Oncopeltus) Embryos

  1. Thomas C. Kaufman1
  1. Department of Biology, Indiana University, Bloomington, IN 47405, USA
  1. ↵1Corresponding author (kaufman{at}indiana.edu)

INTRODUCTION

In this protocol, blastoderm- or germband-stage large milkweed bug (Oncopeltus) embryos are collected, fixed in paraformaldehyde, and dissected from the yolk mass. Germband-stage embryos are stained with SYTOX Green, a fluorescent DNA stain, to facilitate dissection.

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  1. doi:10.1101/pdb.prot5261 Cold Spring Harb Protoc 2009: pdb.prot5261-
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