Protocol

In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana

  1. Bas J. Zwaan
  1. Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands
  1. ↵1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)
This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

The modified in situ hybridization protocol described here can be used to localize RNA transcripts in developing tissues of the African butterfly Bicyclus anynana, including larval and pupal wings and embryos.

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  1. doi:10.1101/pdb.prot5208 Cold Spring Harb Protoc 2009: pdb.prot5208-
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