Protocol

Bradford Assay

  1. David Lane
This protocol was adapted from “Protein Techniques,” Appendix II, in Using Antibodies. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

INTRODUCTION

This assay is used for protein quantitation. It is relatively accurate for most proteins, except for small basic polypeptides such as ribonuclease or lysozyme. It is also hampered by detergent concentrations over ~0.2%.

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This Article

  1. doi:10.1101/pdb.prot4644 Cold Spring Harb Protoc 2006: pdb.prot4644-
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