Fixing Caenorhabditis elegans in Paraformaldehyde

Ed Harlow; David Lane

doi:10.1101/pdb.prot4522

Protocol

Fixing Caenorhabditis elegans in Paraformaldehyde

Ed Harlow and
David Lane

This protocol was adapted from “Staining Tissues,” Chapter 6, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

INTRODUCTION

Another common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms.