Protocol

Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform

  1. Michael Snyder1
  1. Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

    Abstract

    Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Illumina TruSeq Exome Enrichment platform, which captures ∼62 Mb of the human exonic regions using 95-base DNA probes. In addition to covering the RefSeq and Ensembl coding sequences, the enriched sequences also include ∼28 Mb of RefSeq untranslated regions (UTR). The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

    Footnotes

    • ↵1 Correspondence: mpsnyder{at}stanford.edu

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    1. Published in Advance March 11, 2015, doi: 10.1101/pdb.prot084863 Cold Spring Harb Protoc 2015: pdb.prot084863- © 2015 Cold Spring Harbor Laboratory Press
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