Protocol

Microdissection of Drosophila Polytene Chromosomes for DOP-PCR

  1. Abby F. Dernburg
Adapted from Drosophila Protocols (ed. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.

Abstract

This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).

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  1. doi:10.1101/pdb.prot067298 Cold Spring Harb Protoc 2012: pdb.prot067298- © 2012 Cold Spring Harbor Laboratory Press
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