Corrigendum: Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells
Cold Spring Harb Protoc 2016; doi: 10.1101/pdb.prot090068
When this protocol was first published, the concentrations for the reagents in the recipe entitled “Amino Acid Mix (10×)” were expressed as g/mL, but they should have been expressed as g/L. The authors apologize for this error. The HTML version of the recipe (doi: 10.1101/pdb.rec090308) has been corrected, as has the current PDF version of the protocol. A corrected version of the recipe is also included below.
Amino acid or nucleobase | Concentration (10×) |
---|---|
Adenine sulfate | 0.4 g/L |
Uracil | 0.2 g/L |
l-Tryptophan | 0.4 g/L |
l-Histidine hydrochloride | 0.2 g/L |
l-Arginine hydrochloride | 0.2 g/L |
l-Tyrosine | 0.3 g/L |
l-Leucine | 0.6 g/L |
l-Lysine hydrochloride | 0.3 g/L |
l-Phenylalanine | 0.5 g/L |
l-Glutamic acid | 1.0 g/L |
l-Asparagine | 1.0 g/L |
l-Valine | 1.5 g/L |
l-Threonine | 2.0 g/L |
l-Serine | 3.75 g/L |
l-Methionine | 0.2 g/L |
Dissolve the amino acids from the list above (except those to be excluded from any drop-out media) in distilled H2O. Filter-sterilize, store at 4°C, and protect from light. |