Parallel Identification of New Genes in Saccharomyces cerevisiae
- Guy Oshiro1,
- Lisa M. Wodicka2,
- Michael P. Washburn3,
- John R. Yates III3,4,
- David J. Lockhart2,5, and
- Elizabeth A. Winzeler1,4,6
- 1Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA; 2Aventa Biosciences Corporation, San Diego, California 92121, USA; 3Torrey Mesa Research Institute, San Diego, California 92121, USA; 4Department of Cell Biology, The Scripps Research Institute, San Diego, California 92121, USA; 5Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California 92037, USA.
Abstract
Short open reading frames (ORFs) occur frequently in primary genome sequence. Distinguishing bona fide small genes from the tens of thousands of short ORFs is one of the most challenging aspects of genome annotation. Direct experimental evidence is often required. Here we use a combination of expression profiling and mass spectrometry to verify the independent transcription of 138 and the translation of 50 previously nonannotated genes in the Saccharomyces cerevisiae genome. Through combined evidence, we propose the addition of 62 new genes to the genome and provide experimental support for the inclusion of 10 previously identified genes.
[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: V. Velculescu. Supplementary material is available online at http://www.genome.org.]
Footnotes
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↵6 Corresponding author.
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E-MAIL winzeler{at}scripps.edu; FAX (858) 784-9860.
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Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.226802.
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- Received December 7, 2001.
- Accepted May 17, 2002.
- Cold Spring Harbor Laboratory Press
