Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes

  1. Anne Grapin-Botton,
  2. Amit R. Majithia, and
  3. Douglas A. Melton1
  1. Department of Molecular and Cellular Biology, and Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA

Abstract

The mechanisms by which the epithelium of the digestive tract and its associated glands are specified are largely unknown. One clue is that several transcription factors are expressed in specific regions of the endoderm prior to and during organogenesis. Pdx-1, for example, is expressed in the duodenum and pancreas and Pdx-1inactivation results in an arrest of pancreatic development after buds formation. Similarly, ngn3 is transiently expressed in the developing pancreas and a knockout results in the absence of endocrine cells. This paper focuses on the question of whether these and other transcription factors, known to be necessary for pancreatic development, are also sufficient to drive a program of pancreatic organogenesis. Using in ovo electroporation of chick embryos, we show that ectopic expression of Pdx-1 or ngn3 causes cells to bud out of the epithelium like pancreatic progenitors. The Pdx-1-expressing cells extinguish markers for other nonpancreatic regions of the endoderm and initiate, but do not complete, pancreatic cytodifferentiation. Ectopic expression of ngn3 is sufficient to turn endodermal cells of any region into endocrine cells that form islets expressing glucagon and somatostatin in the mesenchyme. The results suggest that simple gene combinations could be used in stem cells to achieve specific endodermal tissue differentiation.

Keywords

Footnotes

  • 1 Corresponding author.

  • E-MAIL dmelton{at}biohp.harvard.edu; FAX (617) 495-8557.

  • Article and publication are at www.genesdev.org/cgi/doi/10.1101/gad.846001.

    • Received August 28, 2000.
    • Accepted December 27, 2000.
| Table of Contents

G&D Most Read

View all ...

Life Science Alliance