A protein interaction map of the LSU processome
- 1Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
- 2Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
- 3Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
- 4Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
- Corresponding author: susan.baserga{at}yale.edu
Abstract
Maturation of the large ribosomal subunit (LSU) in eukaryotes is a complex and highly coordinated process that requires the concerted action of a large, dynamic, ribonucleoprotein complex, the LSU processome. While we know that >80 ribosome biogenesis factors are required throughout the course of LSU assembly, little is known about how these factors interact with each other within the LSU processome. To interrogate its organization and architecture, we took a systems biology approach and performed a semi-high-throughput, array-based, directed yeast two-hybrid assay. Assaying 4800 protein–protein interactions, we identified 232 high-confidence, binary-interacting protein pairs, representing a fourfold increase from current knowledge. The resulting LSU processome interactome map has enhanced our understanding of the organization and function of the biogenesis factors within the LSU processome, revealing both novel and previously identified subcomplexes and hub proteins, including Nop4.
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Footnotes
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Supplemental material is available for this article.
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.256370.114.
- Received November 24, 2014.
- Accepted March 4, 2015.
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