Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development
- Matthew A. Inlay1,7,8,
- Deepta Bhattacharya2,7,
- Debashis Sahoo3,
- Thomas Serwold1,
- Jun Seita1,
- Holger Karsunky4,
- Sylvia K. Plevritis5,
- David L. Dill6 and
- Irving L. Weissman1
- 1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA;
- 2Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA;
- 3Department of Electrical Engineering, Stanford University, Stanford, California 94305, USA;
- 4Cellerant Therapeutics, San Carlos, California 94070, USA;
- 5Department of Radiology, Stanford University, Stanford, California 94305, USA;
- 6Department of Computer Science, Stanford University, Stanford, California 94305, USA
-
↵7 These authors contributed equally to this work.
Abstract
Common lymphoid progenitors (CLPs) clonally produce both B- and T-cell lineages, but have little myeloid potential in vivo. However, some studies claim that the upstream lymphoid-primed multipotent progenitor (LMPP) is the thymic seeding population, and suggest that CLPs are primarily B-cell-restricted. To identify surface proteins that distinguish functional CLPs from B-cell progenitors, we used a new computational method of Mining Developmentally Regulated Genes (MiDReG). We identified Ly6d, which divides CLPs into two distinct populations: one that retains full in vivo lymphoid potential and produces more thymocytes at early timepoints than LMPP, and another that behaves essentially as a B-cell progenitor.
Keywords
Footnotes
-
↵8 Corresponding author.
E-MAIL minlay{at}stanford.edu; FAX (650) 498-6255.
-
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1836009.
-
Supplemental material is available at http://www.genesdev.org.
-
- Received June 24, 2009.
- Accepted September 1, 2009.
- Copyright © 2009 by Cold Spring Harbor Laboratory Press
