Transfer and Regulation of Expression of Chimeric Genes in Plants
This extract was created in the absence of an abstract.
Excerpt
Plant gene vectors derived from the Ti plasmid of Agrobactehum tumefaciens (Zambryski et al. 1983) were used to introduce a number of chimeric genes into tobacco plants. The actual genes involved in these studies were chosen to serve as models for investigating the involvement of 5′ upstream sequences and 3′ downstream sequences in the regulation of gene expression in plants.
In particular we wanted to study induction by light, tissue-specific expression of genes, and induction by elevated temperatures. Finally we wanted to test whether controlling elements, such as the Activator from maize, would be active after transfer to heterologous plants.
THE LIGHT-INDUCIBLE CHALCONE SYNTHASE
Light is one of the most important effectors of differentiation and development in higher plants (Mohr 1972; Mohr and Schäfer 1983). For our investigations we have selected the light-inducible enzyme chalcone synthase (CS), one of the key enzymes involved in flavonoid biosynthesis. Flavonoids constitute one of...
