Homologous Pairing Promoted by Ustilago RecI Protein
This extract was created in the absence of an abstract.
Excerpt
Genetic recombination occurs by breakage and reunion of DNA. The recombining DNA molecules are joined through a region of heteroduplex, a molecular splice in which one strand from each parental DNA molecule pairs with its complement. The process leading to formation of the heteroduplex joint has been envisioned as a series of steps whereby DNA molecules are brought into homologous register and strand exchange is begun (Holliday 1964; Meselson and Rad-ding 1975; Szostak et al. 1983). Until a short while ago, suitable assays were not available for biochemical measurements of genetic recombination. For this reason, explanations of heteroduplex formation during recombination relied in large part on genetical observations. Recently, a much clearer picture of the molecular events surrounding heteroduplex formation has emerged (Radding 1982). With the cloning of the recA gene (McEntee and Epstein 1977; Ogawa et al. 1979; Sancar and Rupp 1979) and the purification of its product, the...
