An Escherichia coli Cell-free System That Catalyzes the Repair of Symmetrically Methylated Heteroduplex DNA
This extract was created in the absence of an abstract.
Excerpt
Heteroduplex DNA containing mismatched nucleotides is produced in Escherichia coli during the process(es) of general genetic recombination between two genetically distinct parental DNAs (Holliday 1964) and following certain types of chemical or physical mutagenesis (Drake and Baltz 1964). The repair of heteroduplex DNA to a homoduplex configuration has been used to explain the nonreciprocal recombination of closely linked markers, termed gene conversion, and map expansion (Fincham and Holliday 1970). Holliday first introduced the concept of heteroduplex DNA into a model for genetic recombination as part of a mechanism for explaining gene conversion in fungi. The success of this model has given the suggested intermediate — the Holliday structure — a central position in current mechanisms of general genetic recombination. This subject has been recently reviewed by Fox (1978), Radding (1978), Warner and Tessman (1978), and Potter and Dressler (1982).
The formation of heteroduplex DNA was originally proposed as resulting from...
