The Molecular Organization of the Leech Nervous System
This extract was created in the absence of an abstract.
Excerpt
Hybridoma technology has now been used in many neurobiological studies. The approaches used by different groups can be generally divided into two classes. Monoclonal antibodies have been raised against identified, relatively well-characterized antigens. For example, Lindstrom and his colleagues (Lindstrom et al., this volume) have raised monoclonal antibodies against the acetylcholine receptor (AChR). In this case, the antigen has been purified and its biochemistry, structure, and function are relatively well understood (Karlin et al.; Raftery et al.; Anderson and Blobel; all this volume). Another approach has been to raise monoclonal antibodies against more complex cellular structures. Two neurobiological systems that have been studied in this way are the retina (Barnstable 1980; Trisler et al. 1981) and the neuromuscular junction (Fambrough et al. 1982). In these cases, hybridomas have been used to define molecular components of a complex structure.
Our own work falls into this second type of study. We have...
