Replication of Bacteriophage λ DNA
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Excerpt
Replication of Bacteriophage λ
The mechanism of bacteriophage λ replication has been studied for a long time via genetic and molecular biological approaches (for review, see Skalka 1977). The following features summarize our current understanding:
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Circular DNA molecules of bacteriophage λ enter into the replication cycle. The replicative intermediates are θ forms at an early stage of bacteriophage λ growth (Tomizawa and Ogawa 1969), but they are rolling-circle forms at late stages (Skalka et al. 1972; Bastia et al. 1975; Takahashi 1975). The switch from θ-type replication to rolling-circle-type replication has not been well studied.
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Replication is initiated at a unique site of DNA called ori (Schnös and Inman 1970; Inman 1981).
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Replication proceeds bidirectionally (Schnös and Inman 1970; Inman 1981).
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Two λ-coded initiator proteins, O and P, are required for replication (Ogawa and Tomizawa 1968). There is evidence from genetic studies that ori is the action site of the
