Genetic and Sequencing Studies of the Specificity of Transposition into the lac Region of E. coli
This extract was created in the absence of an abstract.
Excerpt
Although the cardinal property of transposable elements is their ability to translocate from one position on a donor chromosome or plasmid to one of many different positions on the same or a different DNA molecule, a wide range of insertion specificities has been noted for different elements. At one extreme are elements that insert with little recognizable specificity. A well-studied example in this category is phage Mu (Bukhari and Zipser 1972; Daniell et al. 1972). At the other end of the spectrum is the element IS4, which has been observed at only one position in the gal region (Habermann et al. 1979). All 20 occurrences of IS4 in galT are inseparable by genetic methods, and the three that have been sequenced are at the identical nucleotide position. The majority of elements display specificities in between these two extremes. For example, Tn10 also shows a marked preference for certain hotspots, but...
