Transformation of NIH-3T3 Mouse Cells by Avian Retroviral DNAs
This extract was created in the absence of an abstract.
Excerpt
Transfection by retroviral DNAs has been extensively used to investigate the biological activity of genomic DNAs of avian and mammalian retroviruses. We have been interested in the mechanism of transfection by retroviral DNAs because this mechanism may be important both in the interpretation of experimental results and as a factor affecting the utility of DNA transfection techniques.
Previous studies indicated that genetically stable transformation of chicken embryo fibroblasts (CEFs) by DNA of Rous sarcoma virus (RSV) required production of infectious extracellular progeny virus and secondary infection of sensitive cells (Cooper and Okenquist 1978). Therefore, RSV DNA did not stably transform chicken cells by direct integration of the donor DNA into the recipient cell genome. Instead, transfection of chicken cells appeared to proceed by transcription of donor RSV DNA, formation of progeny virus, and secondary infection of cells in the DNA-treated recipient cultures. The requirement for viral replication prior to genetically...
