Analysis of the Sites of Integration of Viral DNA Sequences in Rat Cells Transformed by Adenovirus 2 or SV40
This extract was created in the absence of an abstract.
Excerpt
All cells transformed by adenovirus type 2 (Ad2) or SV40 carry viral DNA sequences. Often these sequences recombine with those of the host and are then passed on to the cells' descendants like any other part of the cellular genome (Sambrook et al. 1968). While a good deal is known of the number of integrated viral segments present in different cell lines and their general organization (Ketner and Kelly 1976; Botchan et al. 1976; Sambrook et al. 1977), nothing is known of the mechanism by which integration occurs, of the nature of the cellular attachment sites, of the exact locations of the integration sites in the viral genome, or of the mechanism by which viral DNA sequences are expressed. Direct access to the three latter problems has recently become possible with the development of techniques to propagate segments of eukaryotic DNA in prokaryotes. In this paper we describe the use...
