Transcription of Xenopus tDNAmet1 and Sea Urchin Histone DNA Injected into the Xenopus Oocyte Nucleus
This extract was created in the absence of an abstract.
Excerpt
Anyone interested in understanding the functional organization of DNA sequences in isolated eukaryotic genes is faced with the problems that in most cases the transcriptional units are poorly defined and that the individual gene units contain much spacer DNA of unknown function. Although it is now possible to sequence any cloned eukaryotic gene by quite rapid methods (Sanger and Coulson 1975; Maxam and Gilbert 1977), the problem remains of how to distinguish between those sequences which are relevant to gene regulation and those which are not.
We are using two kinds of approaches in an attempt to make such a distinction. The first approach is to ask whether we can identify eukaryotic DNA sequences of potential importance by their locations and by the homologies they may possess with comparably located sequences. The 6-kb histone DNA repeat unit of the sea urchin Psammechinus miliaris, over one-third of which by now has...
