Synthesis of Avian RNA Tumor Virus Structural Proteins
This extract was created in the absence of an abstract.
Excerpt
A great deal of recent research on RNA tumor viruses has been concerned both with the intracellular location and replication of virus-specific nucleic acids and with the functional architecture of the virus particle. By comparison, little is known about the synthesis of virus-specific proteins. Host cell growth and protein synthesis continue during oncornavirus infection, and the synthesis of viral proteins constitutes only a small percentage of the total. Avian myeloblastosis virus (AMV)-infected chick embryo fibroblast (CEF) cells, for example, devote only about 1% of total cell protein synthesis to AMV structural proteins. By using an antiserum prepared against detergent-disrupted AMV to selectively precipitate viral polypeptides, the intracellular translation of viral proteins can be probed despite the vast background of cellular synthesis. The polypeptides present in this immune precipitate can then be analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS). Using this technique, we demonstrated (Vogt and Eisenman 1973)...
