Reverse Transcriptase Associated with A-type Particles from Murine Myeloma Cells
This extract was created in the absence of an abstract.
Excerpt
Intracisternal A-type particles are commonly found in a variety of transformed cells, although they have occasionally been observed in normal cells as well (Potter 1972; Wivel and Smith 1971; Calarco and Szollosi 1973). Thus while these particles resemble morphologically the cores of type B and immature type C particles (Provisional Committee for the Nomenclature of Viruses, 1966), their relationship to bona fide tumor viruses remains uncertain. The resolution of this problem is made difficult by the fact that the intracisternal particles apparently do not ordinarily leave the cell and when they are purified from disrupted cells, they are not infectious. Our approach to this question of the viral relatedness of A particles has been to look for virus-specific macromolecules in purified particles. In particular, we felt that if a true “reverse transcriptase” activity could be found in these particles, it would strongly imply a close relationship to the two other...
