The Action of a Restriction Endonuclease on Higher Organism DNA
This extract was created in the absence of an abstract.
Excerpt
The bacterial restriction endonucleases break double-stranded DNA in both strands. Sequence analysis of the termini of the fragments produced by the enzymes shows that the breaks occur within a short defined sequence (Kelly and Smith, 1970). Rather few fragments are produced by the enzymes from simple DNAs, such as bacteriophage DNAs (Edgell et al., 1972) and SV40 DNA (Danna and Nathans, 1971). The fragments can be separated by gel electrophoresis, which enables their size and their yield to be calculated. Such experiments show that there are a few specific break points for the endonucleases in these DNAs and that the breakage at these points is quantitative. These properties suggest a number of uses for the restriction endonucleases for studies of the repeated sequences in higher organism DNA. The experiments described here had three main objectives: (1) to look for regularities in the distribution of the restriction site in repeated sequences;...
