Nucleotide Sequence Analysis of an Enzymatically Synthesized RNA Corresponding to the 5′-Terminal Region of Qβ RNA
This extract was created in the absence of an abstract.
Excerpt
The determination of both the nucleotide sequence of a messenger RNA, and the amino acid sequence of one or more of the proteins for which it codes may clarify several of the remaining problems of translation. A suitable system for such a project is provided by the small RNA-containing bacteriophages. The single-stranded RNA which serves both as genome and messenger is relatively short, consisting of about 3500 nucleotides, and comprises three or four cistrons. However, the difficulty in determining sequences of RNAs of this size has confined previous investigations to the study of oligonucleotides from complete pancreatic or T1 RNase digestions (Weith and Gilham, 1967; de Wachter and Fiers, 1967 and 1969; Bishop et al., 1968; Dahlberg, 1968; Glitz et al., 1968; de Wachter et al., 1968; Min Jou and Fiers, 1969; Fiers et al., this volume). Very recently, large partial nuclease digestion products have been obtained from R17 RNA...
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↵* Present address: Dept. of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin 53706.
