Association of Enzymes with Replicating and Nonreplicating Simian Virus 40 Chromosomes
This extract was created in the absence of an abstract.
Excerpt
Identification and characterization of the proteins that participate in prokaryotic DNA replication have greatly improved our understanding of the biochemistry of DNA replication and have revealed multiple variations in the detailed processes of DNA replication in different systems. In the case of eukaryotic organisms, however, not one protein has been positively identified as required for DNA replication.
Most previous attempts to identify the proteins required for eukaryotic DNA replication have been based on the report by Friedman and Mueller (1968) that a HeLa cell lysate containing intact nuclei and disrupted cytoplasm can carry out replicative DNA synthesis in vitro, that the capacity of the nuclei for replicative DNA synthesis is greatly reduced if the nuclei are separated from the cytoplasm, and that full capacity is restored by adding back cytoplasm.
Because replication proteins that are not tightly bound to replicating DNA will leak out of the nuclei in this aqueous...
