An Antigen Associated with Messenger RNA in a Transformed Hamster Cell Line
This extract was created in the absence of an abstract.
Excerpt
The discovery of the antigen discussed in this paper (X antigen) originated with an attempt to obtain high-titer antisera against SV40 T antigen by inducing tumors in hamsters with SV40-transformed hamster-mouse hybrid cells. HY1A, a line derived by Wiblin (1971) from C13/SV28 (an SV40-transformed BHK line; Wiblin and MacPherson 1972) and 3T3 cells, is tumorigenic in hamsters. A large number (about 106) of HY1A cells are required to induce a tumor, and the tumors so formed remain small and often regress entirely (Wiblin 1971), probably because of T cell-mediated immune response against mouse surface determinants. Hamsters carrying HY1A tumors may elaborate circulating antibodies with at least three distinct specificities. (1) There is an activity against a nucleoplasms antigen present in all SV40-transformed and SV40-infected cells examined and not in polyoma-transformed or untransformed controls. In some respects, this activity is different from that of more convertional anti-T sera (Reed and Stark,...
