The Regulation of lac Operon Transcription by Cyclic Adenosine 3′, 5′-Monophosphate
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Excerpt
Cyclic adenosine 3′, 5′-monophosphate (cyclic AMP) stimulates the synthesis of β-galactosidase and of other inducible enzymes in E. coli, and prevents the repression of inducible enzyme synthesis produced in this organism by glucose (Perlman, deCrombrugghe, and Pastan, 1969; deCrombrugghe et al., 1969). The lowering of cyclic AMP concentration by glucose and other carbon sources (Makman and Sutherland, 1965) appears to be a major factor in the “glucose effect,” or “catabolite repression.” This paper reviews recent work from our laboratory concerning the mechanism by which cyclic AMP regulates the expression of the lac operon. We have found (1) that cyclic AMP stimulates the synthesis of lac messenger RNA (mRNA), (2) that this effect can be demonstrated in a cell-free system, and (3) that a cyclic AMP binding protein, the cylic AMP receptor protein, is required for this action of the nucleotide. In the course of these experiments, various mutants defective in...
