The Position and Orientation of Genes in λ and λ dg DNA
This extract was created in the absence of an abstract.
Excerpt
The molecule of DNA isolated from bacteriophage λ contains some 47,000 base pairs, enough for 40 to 45 genes, each capable of specifying a polypeptide of 40,000 molecular weight. In fact, some 25 genes have been identified and placed on a genetic map of the vegetative phage.
The ability of some of these λ genes to function in the transcription-translation process appears dependent upon this process having already occurred at other λ genes. This hierarchy of control may contain several levels. For example, the product(s) of the immunity region (iλ see Fig. 1) can be thought of as acting at a primary level since it appears to restrict transcription-translation of the λ genome to the immunity region itself (Jacob and Wollman, 1961; Bode and Kaiser, 1965a; Isaacs, Sly, and Echols, 1965). That this repressive action of the immunity substance(s) may be indirect for all but one, or a few genes,...
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↵* Present Address: The Rockefeller Institute, New York, New York.
