In Situ Hybridization in the Plant Kalanchoë daigremontiana

INTRODUCTION

Here we describe in detail the detection of gene expression in plant tissues of Kalanchoë daigremontiana by in situ hybridization analyses. Included are methods for making RNA transcript probes, probe-tissue hybridization, and detection of antisense RNA probes. The in situ hybridization technique is used to determine which cells or group of cells in particular tissue(s) express a gene of interest.