Protocol

Transformation of the Moss Physcomitrella patens Using Direct DNA Uptake by Protoplasts

  1. Ralph S. Quatrano1
  1. Department of Biology, Washington University, St. Louis, MO 63130, USA
    This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

    INTRODUCTION

    This protocol describes how to transform moss (Physcomitrella patens) protoplasts using polyethylene glycol (PEG)-mediated DNA uptake. The transformation rates for direct uptake by protoplasts of DNA with and without genomic sequence (a targeting construct) are typically 10−5 and 10−3, respectively. (These are the frequencies of stable transformants among regenerants surviving the transformation procedure.)

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    1. doi:10.1101/pdb.prot5143 Cold Spring Harb Protoc 2009: pdb.prot5143-
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