Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices
- 1Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada
- 2Integrated Program in Neuroscience, McGill University, 3801 University Street, Montréal, Quebec H3A 2B4, Canada
Abstract
This protocol provides a method for quadruple whole-cell recording to study synaptic plasticity of neocortical connections, with a special focus on spike-timing-dependent plasticity (STDP). It also describes how to morphologically identify recorded cells from two-photon laser-scanning microscopy (2PLSM) stacks.
Footnotes
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↵3 Correspondence: jesper.sjostrom{at}mcgill.ca
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From the Ion Channels collection, edited by Paul J. Kammermeier, Ian Duguid, and Stephan Brenowitz.
- © 2016 Cold Spring Harbor Laboratory Press
