Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines
Adapted from Imaging in Neuroscience: A Laboratory Manual (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
Because core facilities that generate transgenic founder mice for a reasonable fee are now available at most major research institutions, generating new Thy1-XFP transgenic animals (in which XFP stands for any fluorescent protein) is an option even for relatively small laboratories. Here, we provide a protocol for screening offspring of Thy1 transgenic founders. Acute neuromuscular explants are obtained from 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Thy1-driven expression is detected by fluorescence microscopy.
- © 2015 Cold Spring Harbor Laboratory Press
