Multicolor Flow Cytometric Analysis of Immune Cell Subsets in Tumor-Bearing Mice
- 1Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104;
- 2Abramson Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104;
- 3Division of Hematology-Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Abstract
This protocol describes a procedure for obtaining a single-cell suspension from mouse spleen or solid tissue. Once a single-cell suspension is prepared, multicolor flow cytometry is used to identify subsets of immune cells in the samples of interest. This protocol can be used to evaluate the following immune cell populations, defined by the expression of certain cell surface molecules: total leukocytes, T lymphocytes and CD4+ and CD8+ T-cell subsets, B lymphocytes, natural killer cells, dendritic cells, and immature myeloid cells.
Footnotes
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↵4 Correspondence: rhv{at}exchange.upenn.edu
- © 2013 Cold Spring Harbor Laboratory Press
