Immunostaining of Mitotic Chromosomes from Drosophila Larval Brain
Adapted from Drosophila Protocols (ed. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
Good mitotic chromosome preparations are essential for the immunolocalization of chromosomal proteins. Although methanol/acetic acid fixation techniques preserve chromosome morphology very well, they remove a substantial fraction of chromosomal proteins. We have developed fixation/immunostaining procedures, described here, that are suitable for the immunolocalization of proteinaceous components of metaphase chromosomes from larval Drosophila brain cells. These procedures result in good chromosomal quality with minimal removal of proteins.
- © 2011 Cold Spring Harbor Laboratory Press
