JMJ24 targets CHROMOMETHYLASE3 for proteasomal degradation in Arabidopsis
- 1Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065, USA;
- 2Temasek Life Sciences Laboratory, National University of Singapore, 117604 Singapore
- Corresponding author: chua{at}mail.rockefeller.edu
Abstract
H3K9 methylation is usually associated with DNA methylation, and together they symbolize transcriptionally silenced heterochromatin. A number of proteins involved in epigenetic processes have been characterized. However, how the stability of these proteins is regulated at the post-translational level is largely unknown. Here, we show that an Arabidopsis JmjC domain protein, JMJ24, possesses ubiquitin E3 ligase activity. JMJ24 directly targets a DNA methyltransferase, CHROMOMETHYLASE 3 (CMT3), for proteasomal degradation to initiate destabilization of the heterochromatic state of endogenous silenced loci. Our results uncover an additional connection between two conserved epigenetic modifications: histone modification and DNA methylation.
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Footnotes
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Supplemental material is available for this article.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.274647.115.
- Received November 9, 2015.
- Accepted December 18, 2015.
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