Eeny meeny miny moe, catch a transcript by the toe, or how to enumerate eukaryotic transcripts
- 1Institut Jacques Monod, CNRS UMR 7592, University of Paris-Diderot, 75205 Paris France;
- 2Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland
Abstract
In this issue of Genes & Development, Revyakin and colleagues (pp. 1691–1702) measure the relation between individual RNA polymerase II transcription events and transcription factor assembly by counting RNA transcripts retained on the template DNA using single-molecule fluorescence.
Keywords
- single-molecule fluorescence
- Pol II transcription
- preinitiation complex
- reinitiation
- unstructured probes
- surface passivation
Footnotes
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↵3 Corresponding authors
E-mail strick.terence{at}ijm.univ-paris-diderot.fr
E-mail nouria.hernandez{at}unil.ch
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.199349.112.
- Copyright © 2012 by Cold Spring Harbor Laboratory Press