Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome
- 1Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China
- 2Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
- 3Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 0RE, United Kingdom
- Correspondence: sb10031{at}cam.ac.uk; ckkwok42{at}cityu.edu.hk
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↵4 These authors contributed equally to this work.
SUMMARY
RNA G-quadruplex (rG4) secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms.
