The Need for Integrated Approaches in Metabolic Engineering
- 1Joint Bioenergy Institute (JBEI), Emeryville, California 94608
- 2Department of Chemical & Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, California 94720
- 3Department of Bioengineering, University of California, San Diego, California 92093
- 4Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720
- Correspondence: jdkeasling{at}lbl.gov
Abstract
This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the “system” that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.
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